qpcr retrovirus titration kit

qpcr retrovirus titration kit

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the ability to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell kind and ultimately allow you to administration the expression stage between experiments.

Virus Titration Kits

Our Titration Kits current a fast and straightforward method for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and determine viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than totally different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Enjoying playing cards, which enable quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay method, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Gear offers a fast and straightforward method for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

Description

abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be achieved beneath one hour. Designed to ship extreme sensitivity and specificity, the gear ensures minimal non-specific background and better complete effectivity compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally appropriate with most qPCR gadgets.

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C
qPCR and One-Step RT MasterMixes
qPCR and One-Step RT MasterMixes

 

Quick willpower of retroviral titer

Whereas typical titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the an identical day.

 

The way in which it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embody residual plasmid DNA, might be exactly quantified using this gear.

 

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

Retrovirus Packaging Kit

P905 - Ask for price

QuickTiter Retrovirus Quantitation Kit

VPK-120 20 assays
EUR 711
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

Retrovirus Mini Purification Kit

K1307-10
EUR 544

Retrovirus Mini Purification Kit

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Retrovirus Maxi Purification Kit

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Retrovirus Maxi Purification Kit

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Retrovirus Maxi Purification Kit

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M13 Phage Titration ELISA Kit

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EUR 693
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

Retrovirus Booster 50µl

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Retrovirus Booster 200µl

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Retrovirus Booster 400µl

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Retrovirus Stabilizer 50µl

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Retrovirus Stabilizer 200µl

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Retrovirus Stabilizer 400µl

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Retro-SV40 Retrovirus

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EUR 912

Retro-SV40LT Retrovirus

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Lentivirus and Retrovirus Purification Kit

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qPCR Control Template Kit

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Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Lentivirus qPCR Quantification Kit

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EUR 298

QPCR Positive Control Kit

M1126-100
EUR 403

ViraDuctin Retrovirus Transduction Kit (40 Transductions)

RV-200 40 transductions
EUR 363
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

ViraDuctin Retrovirus Transduction Kit (200 Transductions)

RV-201 200 transductions
EUR 780
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

Endogenous retrovirus (replication competent) PCR kit

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Endogenous retrovirus (replication competent) PCR kit

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Endogenous retrovirus (replication competent) PCR kit

PCR-V725-48D 50T
EUR 425.8
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit

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Retrovirus-related Gag Antibody

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iPS Cell Line (Retrovirus)

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Methylamp MS-qPCR Fast Kit

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